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iimveliso

I-Magnetic Bead DNA Extraction Kit yeKhithi yeNgqokelela yeNgqokelela ye-DNA yokuCoca ngokukhawuleza

Inkcazelo emfutshane:

CY-F006-10 (50preps cells)

CYF006-11 (100preps-cells)

I-CY-F006-12 (200preps-cell)

I-CY-F006-20 (50preps-saliva)

I-CY-F006-21 (100preps-saliva)

I-CY-F006-22 (200preps-saliva)

Ukupakishwa kwezinto:

50 abantu / ibhokisi, 100 abantu / ibhokisi, 200 abantu / ibhokisi


Iinkcukacha zeMveliso

Iithegi zeMveliso

Intshayelelo yeMveliso:

I-Iclean DNA Extraction Kit ibonelela ngendlela ekhawulezayo nesebenzayo yokucoca i-DNA (kubandakanya i-genomic, mitochondrial kunye ne-viral DNA) ukusuka kwizicubu ezigciniweyo, amathe, ulwelo lomzimba, kunye ne-buccal, umlomo wesibeleko, iiseli zesikhumba, iseli yebhaktiriya njl.

Isampulu yebhayoloji Iisampulu zinokugcinwa kwindawo yethu yogcino olukhethekileyo ukuya kuthi ga kwiintsuku ezingama-30 kwiqondo lobushushu legumbi ngaphambi kokuba kulungiswe ngaphandle kwelahleko ebonakalayo kwisivuno se-DNA okanye umgangatho (>iintsuku ezingama-30 ukuba ivenkile ikwimeko yokukhenkceza)

Umgangatho ophezulu we-DNA ye-genomic unokuhlanjululwa kwimizuzu ye-15 ngaphandle kwe-phenol / i-chloroform extraction okanye i-alcohol precipitation, kunye ne-avareji ye-DNA isivuno se-8 μg nge-buccal swab.I-DNA ecocekileyo, emalunga ne-20-30 kb, ifanelekile kwizicelo ezisezantsi ezifana ne-PCR okanye ezinye ii-enzymatic reactions.

Izinto eziluncedo kwimveliso

Ukusebenza okuphezulu, ukutsalwa okukodwa kwe-DNA, ukukhutshwa okukhulu kweprotheni yokungcola kunye nezinye izinto eziphilayo kwiiseli.Iziqwenga ze-DNA ezikhutshiweyo zikhulu, zicocekile, zizinzile kwaye zithembeke kumgangatho.

1. Itekhnoloji esekelwe kwi-Magnetic bead yokuhlukanisa i-DNA ye-genomic ngaphandle kwesidingo seekhemikhali ezinobungozi, i-centrifugation, okanye i-vacuum manifolds, i-phenol kunye ne-ethanol precipitation.

I-2.Ukucocwa ngokukhawuleza nangokufanelekileyo kwe-DNA ye-genomic ukusuka kwi-buccal swabs yabantu ngaphantsi kwemizuzu eyi-15 emva kokulungiswa kwesampuli kunye ne-lysis.

I-3.I-lysis elula kunye ne-Proteinase K ngaphandle kwesidingo sayo nayiphi na i-lysis mechanical.

4.Ungcoliseko oluncinci nge-RNA.

I-5.I-DNA ye-genomic ecocekileyo ibonisa ukusebenza okuphuculweyo kwi-downstream kwizicelo ezibandakanya i-PCR.

I-6.Ibandakanya ikiti eyenzelwe ukuqhutyelwa ngokuzenzekelayo kwamanani amaninzi eesampuli kwiipleyiti ze-96-well usebenzisa i-robot yokuphatha ulwelo.

Usetyenziso lweMveliso:

Ukucocwa kunye nokwahlukaniswa kwe-DNA (kubandakanya i-genomic, mitochondrial, ibhaktiriya, i-parasite kunye ne-viral DNA) kwiithishu, amathe, ulwelo lomzimba, kunye ne-buccal, umlomo wesibeleko, iiseli zolusu, iiseli zebhaktheriya, izicubu, i-swabs, i-CSF, ulwelo lomzimba, iiseli zomchamo ezihlanjiweyo. .

Imiyalelo yeMveliso

1. Ngaphambi kokusebenzisa i-nucleic acid extraction reagents

①.Dlulisa isinyibilikisi seproteinase k kumgubo owomileyo oqulethe iproteinase k kwaye udibanise kakuhle.

②.Yongeza i-18ml kunye ne-42ml ye-ethanol epheleleyo kwi-CY3 kunye ne-CY4 yemodeli ye-CY-F006-10 (i-50preps-cells) kunye ne-CY-F006-20 (i-50preps-saliva), kwaye udibanise kakuhle.

③.Yongeza i-36ml kunye ne-84ml i-ethanol epheleleyo kwi-CY3 kunye ne-CY4 yemodeli ye-CY-F006-11 (i-100preps-cells) kunye ne-CY-F006-21 (i-100preps-saliva) kwaye udibanise kakuhle.

 

2. Amanyathelo okutsalwa kweswab:

①Ingqokelela eyomileyo yeSwab, yongeza i-0.6ml ye-CY1 engamanzi, i-10ul proteinase K, xuba kakuhle, uyibeke kwi-incubator yomoya kuma-65 degrees Celsius kwaye ufukamele imizuzu engama-30 (okanye ingqokelela emanzi: ityhubhu yesampulu ye-centrifuge equlethe iswab kunye nesisombululo sokugcina igxininiswe kwindawo 12000 rpm ngomzuzu om-1 , Gcina imvula, susa i-supernatant Dibanisa i-0.6ml CY1 liquid, 10ul proteinase k, xuba kakuhle, uyibeke kwi-incubator yomoya kwi-65 degrees celsius kwaye ufukamele imizuzu engama-30).

②.Susa i-swab kunye ne-centrifuge kwi-12000rpm ye-1min.

③.Khupha yonke i-supernatant kwityhubhu entsha ye-centrifuge kwaye wenze uvavanyo.

④.Yongeza 0.25ml CY2 ulwelo, 10ul amaso kazibuthe* (zanyazanyiswa kakuhle phambi kokusetyenziswa), xuba kakuhle 12min, yibeke kwi-magnetic stand kwaye ukuma 30s, kwaye kufunxa ulwelo.

⑤ Yongeza i-0.6ml ye-CY3 yolwelo, xuba kakuhle i-3min, uyibeke kwi-magnetic stand kwaye uyivumele imele i-30s ukufunxa ulwelo.

⑥.Yongeza i-0.6ml ye-CY4 yolwelo, xuba i-3min, uyibeke kwi-magnetic stand kwaye uyivumele ime i-30s, ifunxe ulwelo.

⑦.Phinda amanyathelo ②⑥

⑧.Yomisa i-10-20min kwiqondo lobushushu begumbi, yongeza i-50ul CY5 yolwelo lwe-elution, xuba kakuhle, uyibeke kwi-magnetic stand kwaye uyivumele imele i-30s, emva koko udlulisele ulwelo kwityhubhu entsha ye-centrifuge.

⑨.Ukulinganisa i-OD

 

3. Inyathelo lokutsalwa kwamathe

① I-Centrifuge kwi-12000rpm ye-1min kunye namathe kunye nomxube wokulondoloza

② Gcina imvula kwaye ususe i-supernatant

③.Yongeza i-0.6ml yolwelo lweCY1 kunye ne-10ul yeproteinase k kuyo, xuba kakuhle, kwaye uyibeke kwi-incubator yomoya kuma-65 degrees Celsius kwaye ufukamele imizuzu engama-30.

④ I-Centrifuge kwi-12000rpm ye-1min, khupha yonke i-supernatant kwi-tube entsha ye-centrifuge, yongeza i-10ul ye-magnetic beads kunye ne-0.25ml CY2, xuba kakuhle i-12min, uyibeke kwi-stand magnetic kwaye uyivumele imele i-30s ukufunxa ulwelo.

⑤ Yongeza i-0.6ml ye-CY3 yolwelo, xuba kakuhle i-3min, uyibeke kwi-magnetic stand kwaye uyivumele imele i-30s ukufunxa ulwelo.

⑥.Yongeza i-0.6ml ye-CY4 yolwelo, xuba i-3min, uyibeke kwindawo yemagnethi kwaye uyiyeke ime i-30s ukufunxa ulwelo.

⑦.Phinda inyathelo ⑥

⑧.Yomisa kwiqondo lokushisa kwi-10-20 imizuzu, yongeza i-50ul ye-CY5 yolwelo lwe-lution, xuba kakuhle, uyibeke kwi-stand magnetic kwaye uyivumele imele i-30s, uze udlulisele ulwelo kwi-tube entsha ye-centrifuge.

⑨.Ukulinganisa i-OD

Qaphela: Ukuba ufuna ukususa i-RNA, unokulungiselela i-RNaseA 10mg / ml: i-solvent (10mM i-acetate ye-sodium: pH5.0), ubilise i-15min, ulungise i-pH 7.5 nge-Tris-Hcl, kwaye ugcine kwi-20 degrees Celsius.

Iimeko zokugcina kunye nexesha lokuqinisekisa

1. Imveliso kufuneka isetyenziswe kwindawo ecocekileyo necocekileyo, iphephe ukungcola, kwaye inobushushu obufanelekileyo.

2. Gcina kwiqondo lokushisa.Iproteinase K kunye namaso azibuthe anokugcinwa kwi-2-8 ° C ixesha elide.

3. Ubomi beshelufu yemveliso: iinyanga ezili-12

Ukulumkela:

1. Le mveliso isetyenziselwa kuphela ukuxilongwa kwe-in vitro.

2. Indawo yokugcina kunye namanyathelo okukhutshwa kufuneka alandele ngokuthe ngqo imiyalelo ekwincwadana.

3. Ukuba ufumanisa ukuba isixa sincinci kakhulu ngexesha lokukhutshwa, unokwandisa ngokufanelekileyo ubungakanani besampulu okanye ukwandisa inani lokutsalwa.

4. I-DNA ekhutshiweyo kufuneka ibe ntsha kwaye ivavanywe kwangethuba.

Qaphela: Le ndlela yokuthutha isetyenziselwa ukuxilongwa kwe-in vitro, kwaye ayinakusetyenziselwa ukusetyenziswa kwangaphakathi okanye ngaphandle ebantwini okanye kwizilwanyana.Ukuba iginyiwe, inokubangela iziganeko ezinzulu;iyacaphukisa amehlo kunye nolusu.Ukuba iphosa ngempazamo emehlweni, hlambulula ngamanzi.Kufuneka kufakwe umoya ngexesha lokusetyenziswa.

Intshayelelo yomvelisi

I-Huachenyang (i-Shenzhen) i-Technology Co., Ltd. igxile kwimveliso ye-swabs emhlambini, i-throat swabs, i-swabs yomlomo, i-swabs yeempumlo, i-swabs yomlomo wesibeleko, i-sponge swabs, iityhubhu zesampuli zentsholongwane, izisombululo zokugcinwa kwentsholongwane.Inamandla athile kushishino.okulungileyo

Sineminyaka engaphezulu kwe-12+ yeminyaka yamava okwenziwa koMveliso kwizinto eziThengiswayo kwezoNyango

I-HCY ithatha umgangatho wemveliso njengento ebalulekileyo yophuhliso lweshishini, ibambelela kwinqununu "yemveliso yodidi lokuqala, iinkonzo zeklasi yokuqala" ngendlela ejikelezayo, ukuphishekela umoya weshishini "wokufuna inyaniso, ukuveliswa kwezinto ezintsha, ubunye kunye nokusebenza kakuhle" .I-HCY iququzelela yonke inkqubo yokuvelisa kunye nokuthengisa ngokuhambelana ngokungqongqo ne-ISO9001 kunye ne-ISO13485 inkqubo yokulawula, kunye nokusebenza okuzinzile kunye nomgangatho othembekileyo.


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